Microbiome:鼻拭子样本或许久可用于婴儿支气管炎的诊断

Microbiome

2018-01-03Article

8.496

影响因子

原标题:入院支气管炎婴儿鼻前孔与鼻咽菌群的关系

① 对入院的815名支气管炎婴儿的鼻拭子和鼻咽吸出样本进行16S rRNA 基因测序;② 个体两个部位的菌群间存在很强的相关性,都含有较高丰度的嗜血杆菌和莫拉克斯氏菌属;③ 但也存在较大差异,葡萄球菌属在鼻拭子样品中相对丰度较高,在鼻咽吸出样本很少,可能是样品处理方法带来的差异,后续还需验证;④ 尽管个体之间鼻拭子和鼻咽吸出样本的处理方法和菌群存在差异,菌群之间也存在大量重叠,表明鼻拭子可以为年幼儿童的诊断提供有用的样本。

鼻前孔 支气管炎 鼻咽

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Title:
The association between anterior nares and nasopharyngeal microbiota in infants hospitalized for bronchiolitis

DOI:
10.1186/s40168-017-0385-0

Abstract & Authors展开

Abstract:
The airway microbiome is a subject of great interest for the study of respiratory disease. Anterior nare samples are more accessible than samples from deeper within the nasopharynx. However, the correlation between the microbiota found in the anterior nares and the microbiota found within the nasopharynx is unknown. We assessed the anterior nares and nasopharyngeal microbiota to determine (1) the relation of the microbiota from these two upper airway sites and (2) if associations were maintained between the microbiota from these two sites and two bronchiolitis severity outcomes. Among 815 infants hospitalized at 17 US centers for bronchiolitis with optimal 16S rRNA gene sequence reads from both nasal swab and nasopharyngeal aspirate samples, there were strong intra-individual correlations in the microbial communities between the two sample types, especially relating to Haemophilus and Moraxella genera. By contrast, we found a high abundance of Staphylococcus genus in the nasal swabs-a pattern not found in the nasopharyngeal samples and not informative when predicting the dominant nasopharyngeal genera. While these disparities may have been due to sample processing differences (i.e., nasal swabs were mailed at ambient temperature to emulate processing of future parent collected swabs while nasopharyngeal aspirates were mailed on dry ice), a previously reported association between Haemophilus-dominant nasopharyngeal microbiota and the increased severity of bronchiolitis was replicated utilizing the nasal swab microbiota and the same outcome measures: intensive care use (adjusted OR 6.43; 95% CI 2.25-20.51; P < 0.001) and hospital length-of-stay (adjusted OR 4.31; 95% CI, 1.73-11.11; P = 0.002). Additionally, Moraxella-dominant nasopharyngeal microbiota was previously identified as protective against intensive care use, a result that was replicated when analyzing the nasal swab microbiota (adjusted OR 0.30; 95% CI, 0.11-0.64; P = 0.01). While the microbiota of the anterior nares and the nasopharynx are distinct, there is considerable overlap between the bacterial community compositions from these two anatomic sites. Despite processing differences between the samples, these results indicate that microbiota severity associations from the nasopharynx are recapitulated in the anterior nares, suggesting that nasal swab samples not only are effective sample types, but also can be used to detect microbial risk markers.

All Authors:
Pamela N Luna,Kohei Hasegawa,Nadim J Ajami,Janice A Espinola,David M Henke,Joseph F Petrosino,Pedro A Piedra,Ashley F Sullivan,Carlos A Camargo,Chad A Shaw,Jonathan M Mansbach

First Authors:
Pamela N Luna

Correspondence:
Chad A Shaw

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